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Book Details :
Published on: 2016-04-14
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Original language: English

This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. Hydrocarbon And Lipid Microbiology Protocols Single Cell ... Hydrocarbon And Lipid Microbiology Protocols Single Cell And Single Molecule Methods Springer Protocols Handbooks Free eBooks Hydrocarbon and lipid ... Hydrocarbon And Lipid Microbiology Protocols Single Cell ... Hydrocarbon And Lipid Microbiology Protocols Single Cell And Single Molecule Methods Springer Protocols Handbooks ... and lipid microbiology protocol Hydrocarbon and ... Hydrocarbon and Lipid Microbiology Protocols - Springer Single-Cell and Single-Molecule Methods ... Springer Protocols Handbooks. 2016. Hydrocarbon and Lipid Microbiology Protocols Hydrocarbon And Lipid Microbiology Protocols Single Cell ... Hydrocarbon And Lipid Microbiology Protocols Single Cell And Single Molecule Methods Springer Protocols ... Protocols Handbooks Keywords: hydrocarbon and ... Hydrocarbon And Lipid Microbiology Protocols Single Cell ... Hydrocarbon And Lipid Microbiology Protocols Single Cell And Single Molecule Methods Springer Protocols Handbooks Hydrocarbon and lipid microbiology protocols ... Hydrocarbon and Lipid Microbiology Protocols : Single-Cell ... Hydrocarbon and Lipid Microbiology Protocols : Single-Cell and ... single-cell and single-molecule approaches ... of hydrocarbon and lipid microbiology.. ... Hydrocarbon and Lipid Microbiology Protocols - Single-Cell ... Single-Cell and Single-Molecule Methods. ... Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally ... Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols - Single-Cell ... Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods. ... Springer Protocols Handbooks Hydrocarbon And Lipid Microbiology Protocols Single Cell ... ... Single Molecule Methods Springer Protocols Handbooks ... lipid microbiology protocols single cell ... protocols single cell hydrocarbon and lipid ... Single Cell Microbial Ecophysiology with Raman-FISH - Springer Hydrocarbon and Lipid Microbiology Protocols ... Single-Cell and Single-Molecule Methods ... 4 Online ISBN 978-3-662-49131-7 Series Title Springer Protocols Handbooks
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